Entry Detail

General Information

Database ID:LDA0003747
Species:Homo sapiens
Confidence Score:0.982
Contents:>> ncRNA Information
>> Regulatory Relationship
>> Disease Information
>> Evidence Support
>> Reference


ncRNA Information

ncRNA Symbol:SPRY4-IT1
Full Name:SPRY4 intronic transcript 1
Species:Homo sapiens
Start Site(bp):142317620End Site(bp):142318322
Gene Summary:This gene produces a non-coding RNA that is upregulated in several different tumors, including melanoma and breast and prostate cancer cells. This transcript may mediate cell growth, proliferation, and apoptosis. It regulates levels of lipin 2, and therefore may be involved in lipid biosynthesis. The primary transcript is cleaved to release a mature product that localizes to the cytoplasm (PMID:25344859). The full length structure of the primary and cleaved transcripts is unclear, and it is possible that the primary transcript is a splice variant of SPRY4 (sprouty RTK signaling antagonist 4). [provided by RefSeq, Feb 2016]
External Links:
Ensembl ID:ENSG00000281881
Entrez Gene:100642175
VEGA ID:OTTHUMG00000189512
UCSC ID:uc063iel.1
RefSeq Accession:NR_131221


Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
miRNA targets:N/A


Disease Information

 Disease OntologyMeSH
Disease ID:DOID:4947D018281
Disease Name:cholangiocarcinomaCholangiocarcinoma
Category:Disease OntologyMeSH
Type:disease of cellular proliferationNeoplasms
Define:A bile duct adenocarcinoma that has_material_basis_in bile duct epithelial cells.A malignant tumor arising from the epithelium of the BILE DUCTS.
Alias:adult primary Cholangiocarcinoma//adult primary cholangiocellular carcinoma//cholangiosarcomaCholangiocarcinomas//Cholangiocellular Carcinoma//Carcinoma, Cholangiocellular//Carcinomas, Cholangiocellular//Cholangiocellular Carcinomas//Extrahepatic Cholangiocarcinoma//Cholangiocarcinoma, Extrahepatic//Cholangiocarcinomas, Extrahepatic//Extrahepatic Cholangiocarcinomas//Intrahepatic Cholangiocarcinoma//Cholangiocarcinoma, Intrahepatic//Cholangiocarcinomas, Intrahepatic//Intrahepatic Cholangiocarcinomas


Evidence Support

Strong Evidence:ChIP//Luciferase reporter gene assay//RIP//RT-qPCR
Weak Evidence:N/A
Prediction Method:N/A



[1] PubMed ID:29642935
Disease Name:cholangiocarcinoma
Sample:CCA tissues and cell lines
Dysfunction Pattern:Regulation [up-regulated]
Validated Method:RT-qPCR//Luciferase reporter gene assay//ChIP//RIP
Description:SPRY4-IT1 was abnormally upregulated in CCA tissues and cells, and this upregulation was correlated with tumor stage and tumor node metastasis (TNM) stage in CCA patients. SPRY4-IT1 overexpression was also an unfavorable prognostic factor for patients with CCA. Additionally, SP1 could bind directly to the SPRY4-IT1 promoter region and activate its transcription. Furthermore, SPRY4-IT1 silencing caused tumor suppressive effects via reducing cell proliferation, migration and invasion; inducing cell apoptosis and reversing the epithelial-to-mesenchymal transition (EMT) process in CCA cells. Mechanistically, enhancer of zeste homolog 2 (EZH2) along with the lysine specific demethylase 1 (LSD1) or DNA methyltransferase 1 (DNMT1) were recruited by SPRY4-IT1, which functioned as a scaffold. Importantly, SPRY4-IT1 positively regulated the expression of EZH2 through sponging miR-101-3p.
Author:Xu Y,Yao Y,Jiang X,Zhong X,Wang Z,Li C,Kang P,Leng K,Ji D,Li Z,Huang L,Qin W,Cui Y
Title:SP1-induced upregulation of lncRNA SPRY4-IT1 exerts oncogenic properties by scaffolding EZH2/LSD1/DNMT1 and sponging miR-101-3p in cholangiocarcinoma.