Entry Detail



General Information

Database ID:LDA0004117
Species:Homo sapiens
Confidence Score:0.9991
Contents:>> ncRNA Information
>> Regulatory Relationship
>> Disease Information
>> Evidence Support
>> Reference

 

ncRNA Information

ncRNA Symbol:HOTAIR
Full Name:HOX transcript antisense RNA
Category:lncRNA
Species:Homo sapiens
Synonyms:HOXC-AS4//HOXC11-AS1//NCRNA00072
Chromosome:12
Strand:-
Coordinate:
Start Site(bp):53962308End Site(bp):53974956
Gene Summary:This gene is located within the Homeobox C (HOXC) gene cluster on chromosome 12 and is co-expressed with HOXC genes. It represses transcription of HOXD genes on chromosome 2 in trans. This gene is shuttled from chromosome 12 to chromosome 2 by a component of Polycomb Repressive Complex 2 (PRC2). This gene interacts with both PRC2 and lysine specific demethylase 1 (LSD1) complexes through its 5' and 3' domains, respectively, and serves as a scaffold to assemble PRC2 and LSD1 complexes to the HOXD gene cluster. It couples H3K27 methylation and H3K4 demethylation for epigenetic silencing of HOXD genes in multiple tissues. This gene is highly expressed in multiple tumors. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Feb 2013]
External Links:
Ensembl ID:ENSG00000228630
HGNC ID:HGNC:33510
Entrez Gene:100124700
VEGA ID:OTTHUMG00000152934
UCSC ID:uc009zne.5
ENA:DQ926657
RefSeq Accession:NR_003716
UniProtKB:N/A

 

Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
HOXC13
12
53938765
53946544
+
HOXC12
12
53954834
53958956
+
HOXC11
12
53973126
53977643
+
HOXC10
12
53985065
53990279
+
AC012531.3
12
53985845
54034888
+
HOXC6
12
53990624
54030823
+
HOXC9
12
53994895
54003337
+
HOXC8
12
54009106
54012362
+
HOXC4
12
54016931
54056030
+
HOXC5
12
54032853
54035358
+
miRNA targets:
miRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
MIR196A2
12
53991738
53991847
+
MIR615
12
54033950
54034045
+
Display:

 

Disease Information

 Disease OntologyMeSH
Disease ID:DOID:2871D016889
Disease Name:endometrial carcinomaEndometrial Neoplasms
Category:Disease OntologyMeSH
Type:disease of cellular proliferationNeoplasms//Female Urogenital Diseases and Pregnancy Complications
Define:A endometrial cancer that is located_in the tissue lining the uterus.Tumors or cancer of ENDOMETRIUM, the mucous lining of the UTERUS. These neoplasms can be benign or malignant. Their classification and grading are based on the various cell types and the percent of undifferentiated cells.
Alias:carcinoma of the Endometrium//endometrioid carcinoma//endometrioid carcinoma of female Reproductive systemEndometrial Neoplasm//Neoplasm, Endometrial//Neoplasms, Endometrial//Endometrial Carcinoma//Carcinoma, Endometrial//Carcinomas, Endometrial//Endometrial Carcinomas//Endometrial Cancer//Cancer, Endometrial//Cancers, Endometrial//Endometrial Cancers//Endometrium Cancer//Cancer, Endometrium//Cancers, Endometrium//Cancer of the Endometrium//Carcinoma of Endometrium//Endometrium Carcinoma//Endometrium Carcinomas//Cancer of Endometrium//Endometrium Cancers

 

Evidence Support

Strong Evidence:CCK-8 assay//Luciferase reporter gene assay//qPCR//qRT-PCR//RIP//RNA pull-down assay//Transwell assay
Weak Evidence:N/A
Prediction Method:N/A

 

Reference

[1] PubMed ID:24285342
Disease Name:endometrial carcinoma
Sample:endometrial tissue
Dysfunction Pattern:Regulation [up-regulated]
Validated Method:qPCR
Description:Our results revealed that HOTAIR expression in the EC tissues was significantly upregulated compared with normal tissues (p<0.001). In addition,we observed a significant association between HOTAIR expression and the EC grade (p<0.05) and lymph node metastasis (p<0.05). Moreover,we found that a higher HOTAIR expression also correlated with the depth of myometrial invasion (p=0.019) and lymphovascular space invasion (p=0.015). More importantly,patients with a higher HOTAIR expression showed significantly poorer overall survival than those with lower HOTAIR expression (p<0.05).
Author:He X,Bao W,Li X,Chen Z,Che Q,Wang H,Wan XP
Year:2014
Title:The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis.
Causality:No

[2] PubMed ID:24758900
Disease Name:endometrial carcinoma
Sample:tissue, cell line (HEC-1A, HEC-1B, Ishikawa, RL-952)
Dysfunction Pattern:Regulation [up-regulated]
Validated Method:qPCR
Description:Our data showed that HOTAIR expression was higher in endometrial cancer cells and tissues than in normal endometrial tissues. HOTAIR expression was closely related to the tumor stage (P = 0.045),myometrial invasion (P = 0.014),and lymph node metastasis (P = 0.033). The down-regulation of HOTAIR resulted in a significant inhibition of cell proliferation,migration,and invasion and in cell cycle arrest at the G0/G1 phase. Furthermore,HOTAIR depletion significantly suppressed the endometrial cancer tumorigenesis in vivo.
Author:Huang J,Ke P,Guo L,Wang W,Tan H,Liang Y,Yao S
Year:2014
Title:Lentivirus-mediated RNA interference targeting the long noncoding RNA HOTAIR inhibits proliferation and invasion of endometrial carcinoma cells in vitro and in vivo.
Causality:Yes

[3] PubMed ID:29466670
Disease Name:endometrial carcinoma
Sample:human EC tissues (n=23) and cell lines (Ishikawa and HEC-1-A)
Dysfunction Pattern:Expression [low expression]
Validated Method:qRT-PCR//CCK-8 assay//RIP//Transwell assay//RNA pull-down assay//Luciferase reporter gene assay
Description:MiR-646 expression was significantly decreased both in human EC tissues (n=23) and cell lines (Ishikawa and HEC-1-A) compared with the control. Moreover, miR-646 expression was negatively related to HOTAIR in human EC tissues (n=23). Our results also showed that miR-646 overexpression considerably attenuated the E2-promoted viability, migration and invasion of Ishikawa and HEC-1-A cells in vitro. In addition, HOTAIR was confirmed to regulate the viability, migration and invasion of EC cells through negative regulating miR-646. More importantly, we also demonstrated that NPM1 was the target of miR-646, and HOTAIR promoted NPM1 expression through interacting with miR-646 in EC cells.
Author:Zhou YX,Wang C,Mao LW,Wang YL,Xia LQ,Zhao W,Shen J,Chen J
Year:2018
Title:Long noncoding RNA HOTAIR mediates the estrogen-induced metastasis of endometrial cancer cells via the miR-646/NPM1 axis.
Causality:Yes