Entry Detail



General Information

Database ID:LDA0004199
Species:Homo sapiens
Confidence Score:0.7311
Contents:>> ncRNA Information
>> Regulatory Relationship
>> Disease Information
>> Evidence Support
>> Reference

 

ncRNA Information

ncRNA Symbol:PCAT1
Full Name:prostate cancer associated transcript 1 (non-protein coding)
Category:lncRNA
Species:Homo sapiens
Synonyms:PCAT-1//PCA1
Chromosome:8
Strand:+
Coordinate:
Start Site(bp):126847055End Site(bp):127021014
Gene Summary:N/A
External Links:
Ensembl ID:ENSG00000253438
HGNC ID:HGNC:43022
Entrez Gene:100750225
VEGA ID:OTTHUMG00000165061
UCSC ID:uc064qdc.1
ENA:HQ605084
RefSeq Accession:NR_045262
UniProtKB:N/A

 

Regulatory Relationship

mRNA targets:N/A
miRNA targets:N/A
Display:N/A

 

Disease Information

 Disease OntologyMeSH
Disease ID:LDA078N/A
Disease Name:extrahepatic cholangiocarcinomaN/A
Category:LDAN/A
Type:N/AN/A
Define:N/AN/A
Alias:N/AN/A

 

Evidence Support

Strong Evidence:Transwell assay
Weak Evidence:N/A
Prediction Method:N/A

 

Reference

[1] PubMed ID:28753454
Disease Name:extrahepatic cholangiocarcinoma
Sample:ECC tissue samples and cell lines
Dysfunction Pattern:Regulation [down-regulated]
Validated Method:Transwell assay
Description:Here, we showed that downregulation of PCAT1 following transfection with silencing RNA reduced ECC cell growth and increased cell apoptosis. Additionally, PCAT1 suppression inhibited ECC cell migration and invasion as determined by transwell assay. Furthermore, we determined that PCAT1 is a competing endogenous for microRNA (miR)-122, with bioinformatics analysis and luciferase-reporter assay results demonstrating that PCAT1 regulated WNT1 expression via miR-122. Moreover, PCAT1 downregulation increased levels of glycogen synthase kinase 3β and significantly decreased β-catenin levels in whole cell lysates and nuclear fractions, indicating that PCAT1 silencing inhibited the Wnt/β-catenin-signaling pathway. We also observed that exogenous expression of WNT1 reversed PCAT1-silencing-induced inhibition of ECC cell growth inhibition.
Author:Zhang F,Wan M,Xu Y,Li Z,Leng K,Kang P,Cui Y,Jiang X
Year:2017
Title:Long noncoding RNA PCAT1 regulates extrahepatic cholangiocarcinoma progression via the Wnt/β-catenin-signaling pathway.
Causality:Yes