Entry Detail



General Information

Database ID:LDA0004393
Species:Homo sapiens
Confidence Score:0.9975
Contents:>> ncRNA Information
>> Regulatory Relationship
>> Disease Information
>> Evidence Support
>> Reference

 

ncRNA Information

ncRNA Symbol:LINC00339
Full Name:long intergenic non-protein coding RNA 339
Category:lncRNA
Species:Homo sapiens
Synonyms:HSPC157
Chromosome:1
Strand:+
Coordinate:
Start Site(bp):22024558End Site(bp):22031223
Gene Summary:N/A
External Links:
Ensembl ID:ENSG00000218510
HGNC ID:HGNC:25011
Entrez Gene:29092
VEGA ID:OTTHUMG00000002757
UCSC ID:uc284mnl.1
ENA:N/A
RefSeq Accession:NR_023918
UniProtKB:N/A

 

Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
HSPG2
1
21822245
21937297
-
CELA3B
1
21977021
21998642
+
CELA3A
1
22001656
22012539
+
CDC42
1
22052627
22092946
+
WNT4
1
22117305
22143969
-
miRNA targets:N/A
Display:

 

Disease Information

 Disease OntologyMeSH
Disease ID:LDA084N/A
Disease Name:glioma vasculogenic mimicry formationN/A
Category:LDAN/A
Type:N/AN/A
Define:N/AN/A
Alias:N/AN/A

 

Evidence Support

Strong Evidence:ChIP//Knockdown//Luciferase reporter gene assay//qRT-PCR//RIP//Western blot
Weak Evidence:N/A
Prediction Method:N/A

 

Reference

[1] PubMed ID:29499931
Disease Name:glioma vasculogenic mimicry formation
Sample:glioma tissue and glioma cell lines
Dysfunction Pattern:Regulation [miR-539-5p/TWIST1/MMPs Axis]
Validated Method:qRT-PCR//Western blot//Knockdown//ChIP//Luciferase reporter gene assay//RIP
Description:Here, we demonstrated that LINC00339 was upregulated in glioma tissue as well as in glioma cell lines. The expression of LINC00339 in glioma tissues was positively correlated with glioma VM formation. Knockdown of LINC00339 inhibited glioma cell proliferation, migration, invasion, and tube formation, meanwhile downregulating the expression of VM-related molecular MMP-2 and MMP-14. Furthermore, knockdown of LINC00339 significantly increased the expression of miR-539-5p. Both bioinformatics and luciferase reporter assay revealed that LINC00339 regulated the above effects via binding to miR-539-5p. Besides, overexpression of miR-539-5p resulted in decreased expression of TWIST1, a transcription factor known to play an oncogenic role in glioma and identified as a direct target of miR-539-5p. TWIST1 upregulated the promoter activities of MMP-2 and MMP-14. The in_vivo study showed that nude mice carrying tumors with knockdown of LINC00339 and overexpression of miR-539-5p exhibited the smallest tumor volume through inhibiting VM formation.
Author:Guo J,Cai H,Liu X,Zheng J,Liu Y,Gong W,Chen J,Xi Z,Xue Y
Year:2018
Title:Long Non-coding RNA LINC00339 Stimulates Glioma Vasculogenic Mimicry Formation by Regulating the miR-539-5p/TWIST1/MMPs Axis.
Causality:Yes