Entry Detail

General Information

Database ID: LDA0000185
Species: Homo sapiens
Confidence Score: 0.985791
Contents: >> ncRNA Information
>> ncRNA Association Statistics
>> Disease Information
>> Disease Association Statistics
>> Evidence Support
>> Reference
Causality: Yes
Clinical Significance: Yes

 


ncRNA Information

Reference Genome Note: GRCh38 for human lncRNAs; GRCm39 for mouse lncRNAs; mRatBN7.2 for rat lncRNAs; hg19 for human circRNAs; mm9 for mouse circRNAs.

ncRNA Symbol:HOTAIR
Full Name:HOX transcript antisense RNA
Category:LncRNA
Species:Homo sapiens
Synonyms:HOXAS|HOXC-AS4|HOXC11-AS1|NCRNA00072
Chromosome:12
Strand:-
Coordinate:
Start Site(bp):53962308End Site(bp):53974956
External Links:
Ensembl ID:ENSG00000228630
Ensembl Transcript ID:N/A
Entrez Gene:100124700.0
NONCODE ID:N/A
RefSeq Accession:N/A

 

ncRNA Association Statistics

Total Associated Disease Number:110   
More Information
Causal Disease Number:68
Network:
Top Causal Diseases:
Carcinoma, Hepatocellular  (Score: 1)
Carcinoma, Hepatocellular  (Score: 1)
Breast Neoplasms  (Score: 1)
Stomach Neoplasms  (Score: 1)
Breast Neoplasms  (Score: 1)
Stomach Neoplasms  (Score: 1)
Carcinoma, Non-Small-Cell Lung  (Score: 1)
Carcinoma, Non-Small-Cell Lung  (Score: 1)
Colorectal Neoplasms  (Score: 1)
Colorectal Neoplasms  (Score: 1)
More Information

 

 

Disease Information

 Disease OntologyMeSH
Disease ID:DOID:4159D012878
Disease Name:skin cancerSkin Neoplasms
Category:Disease OntologyMeSH
Type:Neoplasms
Define:An integumentary system cancer located_in the skin that is the uncontrolled growth of abnormal skin cells.Tumors or cancer of the SKIN.
Alias:CA - skin cancer//malignant neoplasm of skin//melanoma and Non-melanoma skin cancerSkin Neoplasms//Neoplasms, Skin//Neoplasm, Skin//Skin Neoplasm//Cancer of Skin//Skin Cancers//Cancer of the Skin//Skin Cancer//Cancer, Skin//Cancers, Skin

 

Disease Association Statistics

Total Associated ncRNA Number:52   
More Information
Causal ncRNA Number:48
Network:
Top Causal ncRNAs:
HOTAIR  (Score: 0.985791)
HOTAIR  (Score: 0.985791)
PICSAR  (Score: 0.985791)
PICSAR  (Score: 0.985791)
circSEC24A  (Score: 0.731059)
circLARP1B  (Score: 0.731059)
PRECSIT  (Score: 0.731059)
SCARNA2  (Score: 0.731059)
H19  (Score: 0.731059)
EZR‑AS1  (Score: 0.731059)
More Information

 

Evidence Support

Strong Evidence:In Vivo Experiment//ChIP//Western Blot//Transfection//CCK8//qRT-PCR//RIP//FISH//IF//Luciferase Report Assay//Colony Formation Assay//EdU Staining//Transwell Assay
Weak Evidence:

 

Reference

[1] PubMed ID:31649487
Disease Name:Skin Neoplasms
Sample:cell lines
Dysfunction Pattern:Interaction[sponging miR-326]
Validated Method:Western Blot//CCK8//qRT-PCR//RIP//Luciferase Report Assay//EdU Staining//Transwell Assay
Description:HOTAIR expression in CSCC cell lines exhibited an obvious elevation. Furthermore, HOTAIR competitively bound to miR-326, so as to positively modulate miR-326 expression.
Causality:Yes
Causal Description:Cell function analysis revealed that HOTAIR overexpression remarkably facilitated CSCC cell migration, proliferation and EMT process, which were impeded by down-regulation of HOTAIR.
Clinical-realted Application:

[2] PubMed ID:34697449
Disease Name:Skin Neoplasms
Sample:CSCC, CSCC cell lines A431 or SCC13, A431- or SCC13-derived CSCC stem cells
Dysfunction Pattern:Interaction(Sp1/miR-199a )
Validated Method:In Vivo Experiment//ChIP//Western Blot//Transfection//qRT-PCR//FISH//RIP//Luciferase Report Assay//Colony Formation Assay//IF
Description: By measuring its expression using RT-qPCR in CSCC vs. normal tissues, as well as in CSCC cell lines A431 or SCC13, A431- or SCC13-derived CSCC stem cells (CSCSCs), and normal skin fibroblasts (HSFs), we detected higher expression of HOTAIR in CSCC than in normal tissues, in recurrent than in non-recurrent CSCC tissues, in CSCCs and CSCSCs than in HSFs, and particularly, in CSCSCs than in CSCCs. Mechanistically, HOTAIR directly interacted with and up-regulated Sp1. Sp1 then induced DNMT1-mediated promoter methylation and direct transcriptional repression of miR-199a-5p. Targeting Sp1 or DNMT1 further boosted the in vivo anti-tumor and anti-metastasis activities of targeting HOTAIR.
Causality:Yes
Causal Description: Functional assays on colony formation, EdU incorporation, sphere formation, western blot on stem-cell biomarkers, and in vivo models showed that HOTAIR was essential in maintaining multiple stem cell phenotypes of CSCSCs in vitro and in vivo xenograft growth as well as metastasis.
Clinical-realted Application:Kaplan-Meier analysis suggested that higher expression of HOTAIR was positively correlated with worse overall survival of CSCC patients.