| [1] PubMed ID: | 34308844 |
| Disease Name: | Arthritis, Rheumatoid |
| Sample: | PBMCs |
| Dysfunction Pattern: | Expression(lower expressed) |
| Validated Method: | CCK8//Transfection |
| Description: | Compared with the healthy control subjects, RA patients had significantly increased ESR, CRP, RF, anti-CCP, IgA, and C4 levels (P < 0.05) and significantly decreased Linc00638 expression in the PBMCs (P < 0.01). |
| Causality: | Yes |
| Causal Description: | In RA-FLS, overexpression of Linc00638 significantly inhibited while Linc00638 interference obviously enhanced the cell viability. Over-expression of Linc00638 also significantly increased the levels of IL-4 and SOD (P < 0.05) and decreased the expressions of IL-6 and ROS (P < 0.05), while interference of Linc00638 produced the opposite effects in the cells (P < 0.05). |
| Clinical-realted Application: | The area under the receiver-operating characteristic (ROC) curve of Linc00638 was 91.86% with the best cut-off value of 0.74 for diagnosis of RA. Spearman correlation analysis showed that Linc00638 expression level was negatively correlated with age, course of disease, DAS28, ESR, CRP, RF and anti-CCP, and positively correlated with IL-4 and SOD levels (P < 0.05). |
| [2] PubMed ID: | 35545408 |
| Disease Name: | Arthritis, Rheumatoid |
| Sample: | 48 RA patients |
| Dysfunction Pattern: | Expression(lower expressed) |
| Validated Method: | qRT-PCR//ELISA//IF |
| Description: | Compared with the control group, the expression of LINC00638 in the RA group was lower (P<0.01). |
| Causality: | Yes |
| Causal Description: | Compared with siRNA-control group, LINC00638, IL-10, SOD2 and HO-1 in the siRNA-LINC00638 group were significantly decreased (all P<0.01), and TNF-α was significantly increased (P<0.01). |
| Clinical-realted Application: | |
| [3] PubMed ID: | 35759235 |
| Disease Name: | Arthritis, Rheumatoid |
| Sample: | PBMCs, RA-FLS |
| Dysfunction Pattern: | Regulation(Nrf2/HO-1 pathway) |
| Validated Method: | Western Blot//CCK8//qRT-PCR//Flow Cytometry//IF//ELISA//Transwell Assay |
| Description: | LINC00638 expression was found to be significantly decreased in PBMCs of RA patients, and it was negatively correlated with erythrocyte sedimentation rate, interleukin (IL)-17, reactive oxygen species (ROS), and disease activity scores for 28 joints (DAS28). Overexpression of LINC00638 activated the Nrf2/HO-1 pathway, markedly decreased the expressions of IL-6, IL-17, IL-23, ROS, as well as malondialdehyde, increased the total antioxidant capacity, and attenuated the proliferation and migration of RA-FLS, while silencing of LINC00638 reversed these manifestations. |
| Causality: | Yes |
| Causal Description: | Overexpression of LINC00638 activated the Nrf2/HO-1 pathway, markedly decreased the expressions of IL-6, IL-17, IL-23, ROS, as well as malondialdehyde, increased the total antioxidant capacity, and attenuated the proliferation and migration of RA-FLS, while silencing of LINC00638 reversed these manifestations. |
| Clinical-realted Application: | |
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