Entry Detail

General Information

Database ID: LDA0004173
Species: Homo sapiens
Confidence Score: 0.985791
Contents: >> ncRNA Information
>> ncRNA Association Statistics
>> Disease Information
>> Disease Association Statistics
>> Evidence Support
>> Reference
Causality: Yes
Clinical Significance: Yes

 


ncRNA Information

Reference Genome Note: GRCh38 for human lncRNAs; GRCm39 for mouse lncRNAs; mRatBN7.2 for rat lncRNAs; hg19 for human circRNAs; mm9 for mouse circRNAs.

ncRNA Symbol:AFAP1-AS1
Full Name:AFAP1 antisense RNA 1
Category:LncRNA
Species:Homo sapiens
Synonyms:AFAP1-AS|AFAP1AS
Chromosome:4
Strand:+
Coordinate:
Start Site(bp):7754090End Site(bp):7778928
External Links:
Ensembl ID:ENSG00000272620
Ensembl Transcript ID:N/A
Entrez Gene:84740.0
NONCODE ID:N/A
RefSeq Accession:N/A

 

ncRNA Association Statistics

Total Associated Disease Number:60   
More Information
Causal Disease Number:58
Network:
Top Causal Diseases:
Carcinoma, Non-Small-Cell Lung  (Score: 1)
Carcinoma, Non-Small-Cell Lung  (Score: 1)
Breast Neoplasms  (Score: 1)
Stomach Neoplasms  (Score: 1)
Stomach Neoplasms  (Score: 1)
Breast Neoplasms  (Score: 1)
Prostatic Neoplasms  (Score: 0.999893)
Endometriosis  (Score: 0.999893)
Prostatic Neoplasms  (Score: 0.999893)
Endometriosis  (Score: 0.999893)
More Information

 

 

Disease Information

 Disease OntologyMeSH
Disease ID:DOID:9261C538339
Disease Name:nasopharynx carcinomaNasopharyngeal carcinoma
Category:Disease OntologyMeSH
Type:Neoplasms
Define:A pharynx cancer that is located in the nasopharynx, the uppermost region of the pharynx or throat, where the nasal passages and auditory tubes join the remainder of the upper respiratory tract.A carcinoma that originates in the nasopharyngeal EPITHELIUM and includes 4 subtypes: keratinizing squamous cell, nonkeratinizing, basaloid squamous cell, and PAPILLARY ADENOCARCINOMA. It is most prevalent in Southeast Asian populations and is associated with EPSTEIN-BARR VIRUS INFECTIONS. Somatic mutations have been identified in NPCR, BAP1, UBAP1, ERBB2, ERBB3, MLL2, PIK3CA, KRAS, NRAS, and ARID1A genes. OMIM: 607107
Alias:Nasopharyngeal carcinoma//malignant Nasopharyngeal tumor//malignant neoplasm of nasopharynx//nasopharynx cancer

 

Disease Association Statistics

Total Associated ncRNA Number:260   
More Information
Causal ncRNA Number:244
Network:
Top Causal ncRNAs:
ZFAS1  (Score: 1)
ZFAS1  (Score: 1)
DANCR  (Score: 0.999893)
XIST  (Score: 0.999893)
XIST  (Score: 0.999893)
DANCR  (Score: 0.999893)
circZNF609  (Score: 0.985791)
hsa_circ_0008450  (Score: 0.985791)
PVT1  (Score: 0.985791)
circZNF609  (Score: 0.985791)
More Information

 

Evidence Support

Strong Evidence:RNA Pull-Down//Western Blot//Transfection//Wound Healing Assay//qPCR//CCK8//RIP//qRT-PCR//Luciferase Report Assay//Transwell Assay//ChIP
Weak Evidence:

 

Reference

[1] PubMed ID:33330099
Disease Name:Nasopharyngeal carcinoma
Sample:NPC tissues
Dysfunction Pattern:interaction[binds to KAT2B]
Validated Method:RNA Pull-Down//qRT-PCR//RIP//Luciferase Report Assay//ChIP
Description:AFAP1-AS1 is upregulated in NPC and is a poor prognostic indicator for survival in NPC patients. Mechanistic investigations suggested that AFAP1-AS1 binds to KAT2B and promotes acetyltransferase activation at two residues (E570/D610). KAT2B further promotes H3K14 acetylation and protein binding to the bromo domain of TIF1α. Consequently, TIF1α acts as a nuclear transcriptional coactivator of RBM3 transcription, leading to YAP mRNA stabilization and enhanced NPC tumorigenicity.
Causality:Yes
Causal Description:AFAP1-AS1 was required for NPC proliferation in vitro and tumorigenicity in vivo.
Clinical-realted Application:AFAP1-AS1 is upregulated in NPC and is a poor prognostic indicator for survival in NPC patients.

[2] PubMed ID:33400247
Disease Name:Nasopharyngeal carcinoma
Sample:nasopharyngeal carcinoma tissues and cells
Dysfunction Pattern:Interaction(miR-497-5p/CELF1 pathway)
Validated Method:Western Blot//Transfection//Wound Healing Assay//qPCR//CCK8//RIP//Luciferase Report Assay//Transwell Assay//ChIP
Description:In current study, AFAP1-AS1 was found to be up-regulated in nasopharyngeal carcinoma tissues and cells. The interaction between AFAP1-AS1 and miR-497-5p was confirmed. AFAP1-AS1 was demonstrated to regulate CELF1, a target gene of miR-497-5p. Further functional analysis revealed that AFAP1-AS1 knockdown attenuated SP1-induced nasopharyngeal carcinoma progression.
Causality:Yes
Causal Description:AFAP1-AS1 overexpression and knockdown were conducted in nasopharyngeal carcinoma cells. The results proved that AFAP1-AS1 promoted the survival and migration of nasopharyngeal carcinoma cells. Additionally, specificity protein 1 (SP1) was enhanced in nasopharyngeal carcinoma tissues and cells, and induced AFAP1-AS1 expression.
Clinical-realted Application: