Entry Detail

General Information

Database ID: LDA0004187
Species: Homo sapiens
Confidence Score: 0.985791
Contents: >> ncRNA Information
>> ncRNA Association Statistics
>> Disease Information
>> Disease Association Statistics
>> Evidence Support
>> Reference
Causality: Yes
Clinical Significance: Yes

 


ncRNA Information

Reference Genome Note: GRCh38 for human lncRNAs; GRCm39 for mouse lncRNAs; mRatBN7.2 for rat lncRNAs; hg19 for human circRNAs; mm9 for mouse circRNAs.

ncRNA Symbol:ADAMTS9-AS1
Full Name:ADAMTS9 antisense RNA 1
Category:LncRNA
Species:Homo sapiens
Synonyms:-
Chromosome:3
Strand:+
Coordinate:
Start Site(bp):64561346End Site(bp):64590084
External Links:
Ensembl ID:ENSG00000241158
Ensembl Transcript ID:N/A
Entrez Gene:101929335.0
NONCODE ID:N/A
RefSeq Accession:N/A

 

ncRNA Association Statistics

Total Associated Disease Number:18   
More Information
Causal Disease Number:14
Network:
Top Causal Diseases:
Glioma  (Score: 0.985791)
Breast Neoplasms  (Score: 0.985791)
Urinary Bladder Neoplasms  (Score: 0.985791)
Glioma  (Score: 0.985791)
Breast Neoplasms  (Score: 0.985791)
Urinary Bladder Neoplasms  (Score: 0.985791)
Colorectal Neoplasms  (Score: 0.731059)
Carcinoma, Ovarian Epithelial  (Score: 0.731059)
Prostatic Neoplasms  (Score: 0.731059)
Endometriosis  (Score: 0.731059)
More Information

 

 

Disease Information

 Disease OntologyMeSH
Disease ID:D005910
Disease Name:Glioma
Category:MeSH
Type:Neoplasms
Define:Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
Alias:Glioma//Gliomas//Glial Cell Tumors//Glial Cell Tumor//Tumor, Glial Cell//Tumors, Glial Cell//Mixed Glioma//Glioma, Mixed//Gliomas, Mixed//Mixed Gliomas//Malignant Glioma//Glioma, Malignant//Gliomas, Malignant//Malignant Gliomas

 

Disease Association Statistics

Total Associated ncRNA Number:660   
More Information
Causal ncRNA Number:606
Network:
Top Causal ncRNAs:
H19  (Score: 1)
H19  (Score: 1)
MEG3  (Score: 1)
UCA1  (Score: 1)
UCA1  (Score: 1)
GAS5  (Score: 1)
FOXD2-AS1  (Score: 1)
NEAT1  (Score: 1)
MEG3  (Score: 1)
PVT1  (Score: 1)
More Information

 

Evidence Support

Strong Evidence:Western Blot//Wound Healing Assay//CCK8//qRT-PCR//Flow Cytometry//MTT//Luciferase Report Assay//Invasion Assay//Transwell Assay
Weak Evidence:

 

Reference

[1] PubMed ID:34923953
Disease Name:Glioma
Sample:glioma tissues and cells
Dysfunction Pattern:Regulation(Wnt1, β-catenin, c-myc and PCNA)
Validated Method:Western Blot//Wound Healing Assay//CCK8//qRT-PCR//Invasion Assay//Transwell Assay
Description:Using quantitative real-time PCR analysis, we found that ADAMTS9-AS1 was upregulated in glioma tissues and cells in comparison to corresponding controls. Furthermore, we demonstrated that knockdown of ADAMTS9-AS1 suppressed Wnt1, β-catenin, c-myc and PCNA, while upregulating E-cadherin expression.
Causality:Yes
Causal Description:Functionally, depletion of ADAMTS9-AS1 significantly suppressed the proliferation, migration and invasion in glioma cell lines (U251 and U87), as shown via CCK-8 assay, Edu corporation assay, wound healing assay and transwell assay.
Clinical-realted Application:. ADAMTS9-AS1 expression level was correlated to tumor size (p=0.005) and WHO grade (p=0.002). Kaplan-Meier analysis and Cox multivariate analysis showed that ADAMTS9-AS1 could serve as an independent prognostic factor affecting the overall survival of glioma patients.

[2] PubMed ID:36449154
Disease Name:Glioma
Sample:glioma tissues and cell lines
Dysfunction Pattern:Interaction(miR-128 and miR-150/Ras/MAPK and Wnt pathways)
Validated Method:Western Blot//Wound Healing Assay//Flow Cytometry//qRT-PCR//MTT//Luciferase Report Assay
Description:Then, RT-qPCR verified the upregulation of ADAMTS9-AS1 in glioma tissues and cell lines. Furthermore, dual-luciferase assay supported that cytoplasmic ADAMTS9-AS1 is capable of sponging miR-128 and miR-150, which are known as regulators of Ras/MAPK, PI3K, and Wnt pathways. Overall, these results introduced ADAMTS9-AS1 as an oncogene that upregulates Ras/MAPK and Wnt pathways through sponging of the miR-128 and miR-150 in glioma cells.
Causality:Yes
Causal Description:Finally following the ADAMTS9-AS1 overexpression, upregulation of Ras/MAPK and Wnt signaling pathways was verified through western blotting and Top/Fop flash assay, respectively. At the cellular level, ADAMTS9-AS1 overexpression brought about reduced sub-G1 cell population, increased proliferation rate, reduced apoptosis level, increased migration rate, shortened Bax/Bcl2 ratio, induced EMT, and stemness characteristics of transfected cells, detected by flow cytometry, MTT assay, scratch test, and RT-qPCR.
Clinical-realted Application: