| [1] PubMed ID: | 30506548 |
| Disease Name: | Endometriosis |
| Sample: | ectopic endometrial tissues |
| Dysfunction Pattern: | Interaction[ZEB1] |
| Validated Method: | Western Blot//Wound Healing Assay//qRT-PCR//MTT//Luciferase Report Assay//ELISA//IHC//EdU Staining//Transwell Assay |
| Description: | AFAP1-AS1 levels were much higher in ectopic endometrial tissues than that in eutopic tissues.The knockdown of AFAP1-AS1 significantly inhibited expression from promoter site pGL3-P886 of the EMT-related transcription factor ZEB1. The size of subcutaneous tumours in nude mice was significantly reduced after down-regulation of AFAP1-AS1 expression. |
| Causality: | Yes |
| Causal Description: | After knockdown of AFAP1-AS1, the morphology of endometrial epithelial cells varied from spindle fiber shaped to polygon epithelioid and proliferation, migration, and invasion were each inhibited. |
| Clinical-realted Application: | |
| [2] PubMed ID: | 33949053 |
| Disease Name: | Endometriosis |
| Sample: | ESCs |
| Dysfunction Pattern: | Interaction(miR-424-5p/ STAT3/TGF-β/Smad signaling pathway) |
| Validated Method: | Western Blot//Transfection//Flow Cytometry//qRT-PCR//MTT//Luciferase Report Assay//Transwell Assay |
| Description: | AFAP1-AS1 knockdown or miR-424-5p overexpression inhibited proliferation and migration, and promoted apoptosis in ESCs. Moreover, AFAP1-AS1 activated the STAT3/transforming growth factor-β1 (TGF-β1)/Smad2 axis via directly targeting miR-424-5p. |
| Causality: | Yes |
| Causal Description: | In addition, knockdown of AFAP1-AS1 repressed the expression of ki-67 and Bcl-2, and promoted the levels of cleaved caspase-3 and Bax. Furthermore, knockdown of AFAP1-AS1 inhibited the conversion of E-cadherin to N-cadherin and the expression of Snail. |
| Clinical-realted Application: | |
| [3] PubMed ID: | 35513844 |
| Disease Name: | Endometriosis |
| Sample: | Exo of ESCs |
| Dysfunction Pattern: | Interaction(miR-15a-5p/BCL9) |
| Validated Method: | In Vivo Experiment//RNA Pull-Down//IHC//Western Blot//Transfection//CCK8//qRT-PCR//RIP//Luciferase Report Assay//Transwell Assay//Bioinformatics Analysis//IF |
| Description: | The Exo was successfully isolated from ESCs and we observed high expression of AFAP1-AS1 and BCL9 but low expression of miR-15a-5p in EMS. AFAP1-AS1 bound to BCL9, which was targeted by miR-15a-5p in EMS. In vivo experiments in nude mice revealed that inhibition of Exosomal AFAP1-AS1 suppressed migration and invasion of EcESCs through miR-15a-5p/BCL9. |
| Causality: | Yes |
| Causal Description: | Moreover, Exo derived from EcESCs could deliver AFAP1-AS1 to EcESCs and thus promoting proliferation, migration, and invasion of ESCs. |
| Clinical-realted Application: | |
|