| [1] PubMed ID: | 32354224 |
| Disease Name: | Colorectal Neoplasms |
| Sample: | CRC tissues and cell lines |
| Dysfunction Pattern: | Interaction(miR-484) |
| Validated Method: | CCK8//qRT-PCR |
| Description: | pgm5-as1 was upregulated in CRC tissues and cell lines; however, its downregulation contributed to the decreasing of cell viability, growth, migration, and invasion of SW480 and HCT116 cells. Moreover, miR-484 was predicted as the target of pgm5-as1, and the downregulation of pgm5-as1 partially restored the elevated cell viability, growth, migration, and invasion that were induced by the inhibition of miR-484 expression in SW480 and HCT116 cells. |
| Causality: | Yes |
| Causal Description: | pgm5-as1 was upregulated in CRC tissues and cell lines; however, its downregulation contributed to the decreasing of cell viability, growth, migration, and invasion of SW480 and HCT116 cells. |
| Clinical-realted Application: | |
| [2] PubMed ID: | 31619123 |
| Disease Name: | Colorectal Neoplasms |
| Sample: | CRC tissue |
| Dysfunction Pattern: | Expression[lower expressed] |
| Validated Method: | Western Blot//Colony Formation Assay//CCK8//qRT-PCR//RNA-seq//IHC |
| Description: | Then, we showed that the expression levels of PGM5-AS1, B3GALT5-AS1 and PGM5 were significantly downregulated in CRC tissues compared with corresponding normal tissues. |
| Causality: | Yes |
| Causal Description: | Functionally, overexpression of PGM5-AS1 could induce cell apoptosis and cell cycle arrest in CRC. Animal study indicated that PGM5-AS1 overexpression inhibited CRC growth in vivo. |
| Clinical-realted Application: | |
| [3] PubMed ID: | 32767323 |
| Disease Name: | Colorectal Neoplasms |
| Sample: | colorectal cancer tissues and cells |
| Dysfunction Pattern: | interaction[sponging miR-100-5p] |
| Validated Method: | CCK8//qRT-PCR//Luciferase Report Assay//Transwell Assay |
| Description: | We found that both PGM5-AS1 and SMAD4 were downregulated in human colorectal cancer tissues and cells.In this study, we found that lncRNA-PGM5-AS1 was low expressed in human colorectal cancer cells, which could promote tumor proliferation, migration and invasion by serving as a molecular sponge and by modulating the inhibitory effect of miR-100-5p on tumor suppressor gene SMAD4. |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
| [4] PubMed ID: | 34212174 |
| Disease Name: | Colorectal Neoplasms |
| Sample: | CRC patient plasma |
| Dysfunction Pattern: | Expression(lower expressed) |
| Validated Method: | qRT-PCR |
| Description: | lncRNA urothelial carcinoma-associated 1 (UCA1) and lncRNA phosphoglucomutase 5-antisense RNA 1 (PGM5-AS1) were the most significantly up- and down-regulated lncRNAs in CRC patient plasma, respectively. The area under the ROC curve was calculated to be 0.766, 0.754 and 0.798 for UCA1, PGM5-AS1 and the combination of these two lncRNAs, respectively. Moreover, the diagnostic potential of these two lncRNAs was even higher for the early stages of CRC. The combination of UCA1 and PGM5-AS1 enhanced the AUC to 0.832, and when the lncRNAs were used with carcinoembryonic antigen (CEA), the AUC was further improved to 0.874. |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
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