Entry Detail

General Information

Database ID: LDA0005592
Species: Homo sapiens
Confidence Score: 0.731059
Contents: >> ncRNA Information
>> ncRNA Association Statistics
>> Disease Information
>> Disease Association Statistics
>> Evidence Support
>> Reference
Causality: Yes
Clinical Significance: Yes

 


ncRNA Information

Reference Genome Note: GRCh38 for human lncRNAs; GRCm39 for mouse lncRNAs; mRatBN7.2 for rat lncRNAs; hg19 for human circRNAs; mm9 for mouse circRNAs.

ncRNA Symbol:RBM5-AS1
Full Name:RBM5 antisense RNA 1
Category:LncRNA
Species:Homo sapiens
Synonyms:LUST
Chromosome:3
Strand:-
Coordinate:
Start Site(bp):50099603End Site(bp):50100988
External Links:
Ensembl ID:ENSG00000281691
Ensembl Transcript ID:N/A
Entrez Gene:100775107.0
NONCODE ID:N/A
RefSeq Accession:N/A

 

ncRNA Association Statistics

Total Associated Disease Number:8   
More Information
Causal Disease Number:6
Network:
Top Causal Diseases:
Osteosarcoma  (Score: 0.731059)
Carcinoma, Hepatocellular  (Score: 0.731059)
Squamous Cell Carcinoma of Head and Neck  (Score: 0.731059)
Osteosarcoma  (Score: 0.731059)
Carcinoma, Hepatocellular  (Score: 0.731059)
Squamous Cell Carcinoma of Head and Neck  (Score: 0.731059)
More Information

 

 

Disease Information

 Disease OntologyMeSH
Disease ID:DOID:5520D000077195
Disease Name:head and neck squamous cell carcinomaSquamous Cell Carcinoma of Head and Neck
Category:Disease OntologyMeSH
Type:Neoplasms
Define:A head and neck carcinoma that has_material_basis_in squamous cells that line the moist, mucosal surfaces inside the head and neck.The most common type of head and neck carcinoma that originates from cells on the surface of the NASAL CAVITY; MOUTH; PARANASAL SINUSES, SALIVARY GLANDS, and LARYNX. Mutations in TNFRSF10B, PTEN, and ING1 genes are associated with this cancer.
Alias:carcinoma of the head and neck//squamous cell carcinoma of the head and neck//squamous cell carcinomas of head and neckSquamous Cell Carcinoma of Head and Neck//Head And Neck Squamous Cell Carcinomas//Squamous Cell Carcinoma, Head And Neck//Squamous Cell Carcinoma of the Head and Neck//Head and Neck Squamous Cell Carcinoma//HNSCC//Carcinoma, Squamous Cell of Head and Neck//Squamous Cell Carcinoma of the Larynx//Laryngeal Squamous Cell Carcinoma//Squamous Cell Carcinoma of Larynx//Squamous Cell Carcinoma of the Nasal Cavity//Oral Tongue Squamous Cell Carcinoma//Hypopharyngeal Squamous Cell Carcinoma//Oral Squamous Cell Carcinoma//Oral Cavity Squamous Cell Carcinoma//Oral Squamous Cell Carcinomas//Squamous Cell Carcinoma of the Mouth//Oropharyngeal Squamous Cell Carcinoma

 

Disease Association Statistics

Total Associated ncRNA Number:632   
More Information
Causal ncRNA Number:464
Network:
Top Causal ncRNAs:
XIST  (Score: 1)
CYTOR  (Score: 1)
hsa_circ_0001971  (Score: 1)
MALAT1  (Score: 1)
GAS5  (Score: 1)
HOXA11-AS  (Score: 1)
NORAD  (Score: 1)
MEG3  (Score: 1)
NEAT1  (Score: 1)
CYTOR  (Score: 1)
More Information

 

Evidence Support

Strong Evidence:qRT-PCR//ChIP//Luciferase Report Assay//Western Blot
Weak Evidence:

 

Reference

[1] PubMed ID:31869662
Disease Name:Squamous Cell Carcinoma of Head and Neck
Sample:tumor tissues and their matched adjacent normal tissues, cell lines(Tca8113, SCC9, SCC25, CAL27, HN12, HSU3, FADU,NHOK)
Dysfunction Pattern:Interaction[p53;YAP1]
Validated Method:qRT-PCR//Western Blot//Luciferase Report Assay//ChIP
Description:RBM5-AS1 was dramatically Expression[Expression[up-expression]-expression]regulated in OSCC than in the normal tissues (Fig. 1A). We then analyzed the expression of RMB5-AS1 in OSCC cell lines, including Tca8113, SCC9, SCC25, CAL27, HN12, HSU3, FADU, and normal human oral keratinocytes NHOK cell. The results showed that RBM5-AS1 was Expression[Expression[up-expression]-expression]regulated in OSCC comparing to NHOK cells.We also found that RBM5-AS1 regulates the level of miR-1285-3p as a competitive endogenous RNA (ceRNA), therefore regulate the expression level of an oncogene-YAP1, a target of miR-1285-3p.
Causality:Yes
Causal Description: KnockExpression[down-expression] of RBM5-AS1 significantly inhibited cell proliferation compared to the negative control groExpression[Expression[up-expression]-expression] in both cell lines (Fig. 3, A and B). Our results from colony forming assay with Tca8113 and SCC9 cells after siRNA transfection also showed that si-RBM5-AS1#1 and si-RBM5-AS1#2 effectively inhibited colony formation (Fig. 3, C and D).
Clinical-realted Application:Subsequently, we used the chi-square test to determine RBM5-AS1 expression and clinicopathological characteristics correlation in OSCC patients. RBM5-AS1 was closely related to the tumor size, differentiation, TNM stage, and lymph node metastasis, but not to the patient's age, sex, and tumor location (Table 1).