| [1] PubMed ID: | 31601234 |
| Disease Name: | Adenocarcinoma Of Esophagus |
| Sample: | EAC tissues |
| Dysfunction Pattern: | Interaction[YAP1] |
| Validated Method: | qRT-PCR |
| Description: | PVT1 expression was up-regulated in EACs compared with paired BEs, and normal esophageal tissues. Mechanistically, we discovered mutual regulation of PVT1 and YAP1 in EAC cells. Inhibition of PVT1 by PVT1 ASOs suppressed YAP1 expression through increased phosphor-LATS1and phosphor-YAP1 while knockout of YAP1 in EAC cells significantly suppressed PVT1 levels indicating a positive regulation of PVT1 by YAP1. Most importantly, we found that targeting both PVT1 and YAP1 using their specific ASOs led to better antitumor activity in vitro and in vivo. + |
| Causality: | Yes |
| Causal Description: | Effective knockdown of PVT1 in EAC cells using PVT1 ASOs resulted in decreased cell proliferation, invasion, colony formation, tumor sphere formation, and reduced proportion of ALDH1A1 cells. |
| Clinical-realted Application: | High expression of PVT1 was associated with poor differentiation, lymph node metastases, and shorter survival. |
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