| [1] PubMed ID: | 32945470 |
| Disease Name: | capillary hemangioma |
| Sample: | IH tissues |
| Dysfunction Pattern: | regulation[HIF1α/NF-κB signaling pathway] |
| Validated Method: | qRT-PCR//Luciferase Report Assay//Western Blot |
| Description: | Reverse transcription?-quantitative PCR indicated that IH tissues exhibited high expression levels of NEAT1 and hypoxia?inducible factor 1α (HIF1α), and low expression levels of the microRNA (miR)?33a?5p. Collectively, the results revealed that depletion of lncRNA NEAT1 suppressed the tumorigenesis of IH by competitively binding miR‑33a‑5p and thereby stimulating the HIF1α/NF‑κB signaling pathway. |
| Causality: | Yes |
| Causal Description: | Small interfering RNA?mediated depletion of NEAT1 suppressed hemangioma endothelial cell (HemEC) proliferation, migration and invasion. |
| Clinical-realted Application: | |
| [2] PubMed ID: | 35182329 |
| Disease Name: | capillary hemangioma |
| Sample: | IH tissues and cells |
| Dysfunction Pattern: | Interaction(miR-378b/FOSL1 axis) |
| Validated Method: | In Vivo Experiment//Western Blot//MeRIP//Transfection//Migration Assay//CCK8//RIP//qRT-PCR//Luciferase Report Assay//Cell Apoptosis Assay//Invasion Assay//Transwell Assay |
| Description: | ALKBH5, lncRNA NEAT1 and FOLS1 expression was elevated in IH tissues, while miR-378b was downregulated.ALKBH5 might have great potential as therapeutic target for IH, since ALKBH5 silencing suppressed IH progression by regulation of the NEAT1/miR-378b/FOSL1 axis. |
| Causality: | Yes |
| Causal Description: | In addition, miR-378b was the target of lncRNA NEAT1, and its overexpression reversed the promotion effect of lncRNA NEAT1 overexpression on IH cell tumor-like behaviors. Moreover, FOLS1 was the target of miR-378b, and its overexpression reversed the inhibitory effect of miR-378b overexpression on IH cell tumor-like behaviors in vitro. |
| Clinical-realted Application: | |
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