| [1] PubMed ID: | 31497917 |
| Disease Name: | Stomach Neoplasms |
| Sample: | gastric adenocarcinoma and paired normal tissues |
| Dysfunction Pattern: | Interaction[MCL-1;miR-29c-3p ] |
| Validated Method: | qRT-PCR//RIP//Western Blot |
| Description: | Then, the expression of lncRNA MYOSLID in GC tissues was detected by real‐time PCR and found that the expression of lncRNA MYOSLID was higher in GC tissues than in matched non‐tumour tissues (n = 75, P < .0001, Figure Figure1C).1C). We found for the first time that the expression of lncRNA MYOSLID was significantly Expression[Expression[up-expression]-expression]-regulated in GC tissues, and the Expression[Expression[up-expression]-expression]-regulation of lncRNA MYOSLID in GC was correlated with tumour size, AJCC stage, depth of invasion and survival time. In addition, apoptosis and growth arrest can be induced in vitro after knockExpression[down-expression] of lncRNA MYOSLID, which inhibits tumorigenesis in mouse xenografts in vivo. Further in-depth studies revealed that lncRNA MYOSLID acts as a ceRNA of miR-29c-3p, resulting in de-repression of its Expression[down-expression]stream target gene MCL-1. |
| Causality: | Yes |
| Causal Description: | In addition, apoptosis and growth arrest can be induced in vitro after knockExpression[down-expression] of lncRNA MYOSLID, which inhibits tumorigenesis in mouse xenografts in vivo. Further in-depth studies revealed that lncRNA MYOSLID acts as a ceRNA of miR-29c-3p, resulting in de-repression of its Expression[down-expression]stream target gene MCL-1. |
| Clinical-realted Application: | As shown in Table S1, high lncRNA MYOSLID expression was associated with patients age (P = .018), larger tumour size (P = .001), invasion‐related depth (P = .010) and AJCC staging (P = .001), while lncRNA MYOSLID was no significant correlation between expression and other factors including gender (P = 1.000). We also examined the association between lncRNA MYOSLID expression levels and prognosis in patients with GC. Kaplan‐Meier survival analysis showed that patients with higher lncRNA MYOSLID levels had shorter overall survival than patients with lower lncRNA MYOSLID levels (Figure (Figure1D).1D). In addition, lncRNA MYOSLID was significantly overexpressed in GC cell lines compared with human normal gastric epithelial cells (GES‐1) (Figure (Figure1E).1E). These data suggested that IncRNA MYOSLID is involved in the pathogenesis of GC. |
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