| [1] PubMed ID: | 34808393 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | NAFLD cellular model |
| Dysfunction Pattern: | Interaction( miR-139-5p/c-Jun/SREBP-1c pathway) |
| Validated Method: | Western Blot//Transfection//qRT-PCR//Luciferase Report Assay//Oil Red O Staining |
| Description: | NEAT1, c-Jun and SREBP1c expression was markedly elevated, while miR-139-5p expression was reduced in the NAFLD cellular model. NEAT1 knockdown restrained lipid accumulation in the NAFLD cellular model by directly targeting miR-139-5p. Moreover, miR-139-5p overexpression suppressed lipid accumulation by directly suppressing c-Jun expression. In addition, c-Jun silencing suppressed lipid accumulation by directly targeting SREBP1c. Finally, miR-139-5p inhibition mitigated the inhibitory effect of sh-NEAT1 on lipid accumulation. |
| Causality: | Yes |
| Causal Description: | NEAT1 knockdown restrained lipid accumulation in the NAFLD cellular model by directly targeting miR-139-5p. Moreover, miR-139-5p overexpression suppressed lipid accumulation by directly suppressing c-Jun expression. In addition, c-Jun silencing suppressed lipid accumulation by directly targeting SREBP1c. Finally, miR-139-5p inhibition mitigated the inhibitory effect of sh-NEAT1 on lipid accumulation. |
| Clinical-realted Application: | |
| [2] PubMed ID: | 34652536 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | NAFLD patients |
| Dysfunction Pattern: | Interaction(miR-212-5p/GRIA3 ) |
| Validated Method: | Western Blot//qRT-PCR//RIP//Luciferase Report Assay//Oil Red O Staining//ChIP |
| Description: | LncRNA NEAT1 and GRIA3 was upregulated, while miR-212-5p was downregulated in NAFLD patients. ChIP indicated enrichment of H3K27 on NEAT1 promoter. Luciferase results indicated direct interaction of NEAT1/miR-212-5p (which was confirmed by RIP) and miR-212-5p/GRIA3. |
| Causality: | Yes |
| Causal Description: | FFA promoted lncRNA NEAT1 and GRIA3 expression while suppressing miR-212-5p and promoted lipid accumulation as indicated by increased oil red O staining and FAS and ACC expression. |
| Clinical-realted Application: | |
| [3] PubMed ID: | 28771824 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | |
| Dysfunction Pattern: | Regulation(mTOR/S6K1 signaling pathway) |
| Validated Method: | In Vivo Experiment//Western Blot |
| Description: | The level of NEAT1 expression and the mRNA levels of ACC and FAS were obviously enhanced in NAFLD model both in vivo and in vitro. Knockdown of NEAT1 could also reduce the elevation of ACC and FAS induced by FFA in liver cells. Moreover, inhibition of mTOR/S6K1 pathway presented with the same effect with knockdown of NEAT1 on the expression of ACC and FAS mRNA levels. |
| Causality: | Yes |
| Causal Description: | The injection of si-NEAT1 lentivirus was performed to treat NAFLD of rats and the obvious efficacy for NAFLD rats was achieved. |
| Clinical-realted Application: | |
| [4] PubMed ID: | 31239155 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | hepacytes |
| Dysfunction Pattern: | Regulation[AMPK/SREBP-1 signaling] |
| Validated Method: | qRT-PCR//Western Blot |
| Description: | NEAT1 and miR-140 are upregulated in hepacytes under the NAFLD conditions.The NEAT1-miR-140 axis play a crucial role in modulation of NAFLD via inactivation of AMPK/SREBP1 signaling. This study may provide a novel insight for the treatment of NAFLD. |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
| [5] PubMed ID: | 31957704 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | cell lines |
| Dysfunction Pattern: | Regulation[miR-506/GLI3 axis ] |
| Validated Method: | CCK8//qRT-PCR//Luciferase Report Assay//Western Blot |
| Description: | NEAT1 was upregulated while miR-506 was downregulated in the progression of NAFLD. lncRNA NEAT1 could regulate fibrosis, inflammatory response, and lipid metabolism via the miR-506/GLI3 axis as a ceRNA, which is a novel mechanistic role in the regulation of NAFLD. |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
| [6] PubMed ID: | 35559416 |
| Disease Name: | Non-alcoholic Fatty Liver Disease |
| Sample: | PBMCs |
| Dysfunction Pattern: | Expression(highly expressed) |
| Validated Method: | ELISA |
| Description: | The relative expressions of lncRNA NEAT1 in the PBMCs of the NAFLD group were remarkably higher than they were in the control group (P<0.05).The lncRNA NEAT1 expressions in the NAFLD patients presented a remarkable positive correlation with the ALT, GGT, TC, and TG levels (P<0.05). The HEPC levels were positively correlated with the ALT, GGT, TC, and TG levels in the NAFLD patients (P<0.05), and the lncRNA NEAT1 expressions in the peripheral blood had a positive correlation with HEPC (P<0.05). |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
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