| [1] PubMed ID: | 33594304 |
| Disease Name: | bronchopulmonary dysplasia |
| Sample: | |
| Dysfunction Pattern: | Interaction(miR-206) |
| Validated Method: | In Vivo Experiment//Transfection//Tunel//qRT-PCR//RIP//Luciferase Report Assay//ELISA |
| Description: | In our study, we first found that serum MALAT1 was up-regulated in neonatal BPD and severe BPD. Further, through receiver operating characteristic curve (ROC) analysis, it was found that the area under the curve of MALAT1 for differentiating neonatal BPD from severe BPD was 0.943 and 0.866, respectively. Pathological evaluation confirmed that down-regulating MALAT1 or up-regulating miR-206 might improve the pathological condition of BPD. Furthermore, we also found that MALAT1 has a targeted relationship with miR-206, and miR-206 is down-regulated in BPD in vivo and in vitro. Down-regulating miR-206 could also eliminate the anti-BPD effect after knocking down MALAT1. |
| Causality: | Yes |
| Causal Description: | Then, we established BPD models in vivo and in vitro with C57BL/6J mice and BEAS-2B cells, and found that MALAT1 was also highly expressed in them and increased with the induction time of the models. Obvious inflammatory response, oxidative stress and up-regulated apoptosis were observed in BPD models in vivo and in vitro. However, after MALAT1 knockdown treatment, the above abnormal phenomena were alleviated to varying degrees. |
| Clinical-realted Application: | |
| [2] PubMed ID: | 33708512 |
| Disease Name: | Bronchopulmonary Dysplasia |
| Sample: | BPD premature infants |
| Dysfunction Pattern: | Interaction(Keap1/Nrf2 signal pathway) |
| Validated Method: | qRT-PCR//Transfection//Cell Apoptosis Assay//Western Blot |
| Description: | The results showed that, compared to the control group, the expression of MALAT1 increased significantly, and AIF decreased substantially in BPD premature infants. In the A549 hyperoxic lung injury model, compared with the air group, the expression of MALAT1 in the hyperoxia group decreased markedly, while the expression of Keap1 and Nrf2 increased considerably. Furthermore, compared with the control plasmid transfection air group (NC group), the expression of Keap1 and Nrf2 increased significantly in the small interfering RNA (siRNA) group. |
| Causality: | No |
| Causal Description: | |
| Clinical-realted Application: | |
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